Species:Escherichia coli
Group:K12
Strain designation:DP50
Accession number:LMBP 8014
Depositor:M.Mergeay1 and A.Provoost1
1 SCK.CEN,Mol,Belgium
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Other culture collection numbers:CGSC 6493
Medium:LB-Lennox+thymine(25μg/ml)+diaminopimelic acid
(100μg/ml)
Selection marker:/
Temperature:37°C
Genotype:F-,fhuA53,dapD8,lacY1 orΔ(cod-lacI)6,glnX44(AS),Δ(gal-uvrB)47,λ-,gyrA29,ΔthyA57,hsdS3(Source:CGSC 6493)
Phenotype:NalR
Properties:This strain is used for propagation of phage lambda and phage lambda Charon.
Further information:The strain number in the SCK collection is CM0241.
The nalidixic acid resistence could not be experimentally confirmed.
Original reference:Leder et al.,Science 196(1977),175-177[PMID:322278]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 DP50(LMBP 8014)is available at BCCM/GeneCorner.It was deposited by M.Mergeay and A.Provoost and was published in Leder et al.,1977.
Group:K12
Strain designation:DP50
Accession number:LMBP 8014
Depositor:M.Mergeay1 and A.Provoost1
1 SCK.CEN,Mol,Belgium
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Other culture collection numbers:CGSC 6493
Medium:LB-Lennox+thymine(25μg/ml)+diaminopimelic acid
(100μg/ml)
Selection marker:/
Temperature:37°C
Genotype:F-,fhuA53,dapD8,lacY1 orΔ(cod-lacI)6,glnX44(AS),Δ(gal-uvrB)47,λ-,gyrA29,ΔthyA57,hsdS3(Source:CGSC 6493)
Phenotype:NalR
Properties:This strain is used for propagation of phage lambda and phage lambda Charon.
Further information:The strain number in the SCK collection is CM0241.
The nalidixic acid resistence could not be experimentally confirmed.
Original reference:Leder et al.,Science 196(1977),175-177[PMID:322278]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 DP50(LMBP 8014)is available at BCCM/GeneCorner.It was deposited by M.Mergeay and A.Provoost and was published in Leder et al.,1977.
