Species:Escherichia coli
Group:B
Strain designation:Evo21(DE3)
Accession number:LMBP 12941
Depositor:Dr S.Heyde1 and Prof.Dr M.N?rholm1.It was constructed by Dr S.Heyde1.
1 The Novo Nordisk Foundation Center for Biosustainability,Technical University of Denmark,Horsholm,Denmark
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:-
Temperature:28°C
Genotype:F-ompT gal dcm lon hsdSB(rB-mB-)λ(DE3[lacI lacUV5-T7gene1 ind1 sam7 nin5])ΔlacIΔlacZ rne::IS1
Phenotype:-
Properties:This strain is intended for protein production.The strain contains DE3,aλprophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq.The lacI and lacZ genes have been deleted.
Further information:The genome sequence of this strain corresponds with EMBL project number PRJNA741995.
Original reference:Heyde et al.,Commun Biol.4(2021),963[PMID:34385596][DOI:10.1038/s42003-021-02493-4]
Restricted use:-BCCM MTA
-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Refer in your Materials and Methods:
Escherichia coli B Evo21(DE3)(LMBP 12941)is available at BCCM/GeneCorner.It was deposited by Dr S.Heyde and Prof.Dr M.N?rholm and was published in Heyde et al.,2021.
Group:B
Strain designation:Evo21(DE3)
Accession number:LMBP 12941
Depositor:Dr S.Heyde1 and Prof.Dr M.N?rholm1.It was constructed by Dr S.Heyde1.
1 The Novo Nordisk Foundation Center for Biosustainability,Technical University of Denmark,Horsholm,Denmark
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:-
Temperature:28°C
Genotype:F-ompT gal dcm lon hsdSB(rB-mB-)λ(DE3[lacI lacUV5-T7gene1 ind1 sam7 nin5])ΔlacIΔlacZ rne::IS1
Phenotype:-
Properties:This strain is intended for protein production.The strain contains DE3,aλprophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq.The lacI and lacZ genes have been deleted.
Further information:The genome sequence of this strain corresponds with EMBL project number PRJNA741995.
Original reference:Heyde et al.,Commun Biol.4(2021),963[PMID:34385596][DOI:10.1038/s42003-021-02493-4]
Restricted use:-BCCM MTA
-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Refer in your Materials and Methods:
Escherichia coli B Evo21(DE3)(LMBP 12941)is available at BCCM/GeneCorner.It was deposited by Dr S.Heyde and Prof.Dr M.N?rholm and was published in Heyde et al.,2021.
