Species:Escherichia coli
Group:K12
Strain designation:FHK12
Accession number:LMBP 11202
Depositor:Dr A.Fulara1 2 and Prof.Dr S.Savvides1 2
1 Department of Biochemistry and Microbiology,Ghent University,Ghent,Belgium
2 VIB-UGent Center for Inflammation Research,VIB,Ghent,Belgium
It was received from Prof Dr D.Langosch3
3 Center for Integrated Protein Science Munich CIPSM,Technical University of Munich,Germany
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:ampicillin(100μg/ml)
Temperature:37°C
Genotype:F'lacIq lacZΔM15 proA+B+araΔ(lac-proAB)rpsL(?80dlacZΔM15)attB::(ctx::lacZ)(source:Kolmar et al.,1995)
Phenotype:ampR strR
Properties:The ctx::lacZ fusion integrated at the attB site is tagged with the ampicillin resistance gene.
Further information:This FHK12 strain was constructed as follows:
First FHK11 was constructed from strain JM83(Yanish-Perron et al.,1985)by chromosomal integration of the lacZ gene under control of the V.cholerae ctxAB promoter from plasmid pLDR10-ctx::lacZ at the phageλattB site(Diederich et al.,1992).
FHK12 was subsequently constructed from FHK11 by F-duction(Miller,1972)with BMH71-18(source B.Müller-Hill).
The streptomycin resistance has been experimentally confirmed by BCCM/GeneCorner.
Original reference:Kolmar et al.,EMBO J.14(1995),3895-3904[PMID:7664730]
Related reference:Kolmar et al.,Biol.Chem.Hoppe-Seyler 375(1994),61-69[PMID:8003258]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli FHK12(LMBP 11202)is available at BCCM/GeneCorner.It was deposited by Dr A.Fulara and Prof.Dr S.Savvides and was published in Kolmar et al.,1995.
Group:K12
Strain designation:FHK12
Accession number:LMBP 11202
Depositor:Dr A.Fulara1 2 and Prof.Dr S.Savvides1 2
1 Department of Biochemistry and Microbiology,Ghent University,Ghent,Belgium
2 VIB-UGent Center for Inflammation Research,VIB,Ghent,Belgium
It was received from Prof Dr D.Langosch3
3 Center for Integrated Protein Science Munich CIPSM,Technical University of Munich,Germany
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:ampicillin(100μg/ml)
Temperature:37°C
Genotype:F'lacIq lacZΔM15 proA+B+araΔ(lac-proAB)rpsL(?80dlacZΔM15)attB::(ctx::lacZ)(source:Kolmar et al.,1995)
Phenotype:ampR strR
Properties:The ctx::lacZ fusion integrated at the attB site is tagged with the ampicillin resistance gene.
Further information:This FHK12 strain was constructed as follows:
First FHK11 was constructed from strain JM83(Yanish-Perron et al.,1985)by chromosomal integration of the lacZ gene under control of the V.cholerae ctxAB promoter from plasmid pLDR10-ctx::lacZ at the phageλattB site(Diederich et al.,1992).
FHK12 was subsequently constructed from FHK11 by F-duction(Miller,1972)with BMH71-18(source B.Müller-Hill).
The streptomycin resistance has been experimentally confirmed by BCCM/GeneCorner.
Original reference:Kolmar et al.,EMBO J.14(1995),3895-3904[PMID:7664730]
Related reference:Kolmar et al.,Biol.Chem.Hoppe-Seyler 375(1994),61-69[PMID:8003258]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli FHK12(LMBP 11202)is available at BCCM/GeneCorner.It was deposited by Dr A.Fulara and Prof.Dr S.Savvides and was published in Kolmar et al.,1995.
