Species:Escherichia coli
Group:K12
Strain designation:GM2198
Accession number:LMBP 4435
Depositor:Prof.Dr E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:/
Temperature:37
Genotype:F-thr-1 araC14 leuB6(Am)fhuA31 lacY1 tsx-78 glnX44(AS)galK2(Oc)λ-dcm-6 hisG4(Oc)rpsL136 dam-13::Tn9 xylA5 mtl-1 thiE1(Source:CGSC 6623)
Phenotype:StrR CamR
Properties:This is a dam-and dcm-strain(experimentally verified at the Department of Biomedical Molecular Biology,Ghent University,Belgium).
Further information:-
Original reference:Marinus et al.,Mol.Gen.Genet.192(1983),288-289[PMID:6316110]
Restricted use:-BCCM MTA
-Acknowledge Dr Martin Marinus as originator of the strain
Refer in your Materials and Methods:
Escherichia coli K12 GM2198(LMBP 4435)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Marinus et al.,1983.