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　　 　　Species:Escherichia coli

　　Group:K12

　　Strain designation:HB94

　　Accession number:LMBP 7992

　　Depositor:M.Mergeay1 and A.Provoost1

　　1 SCK.CEN,Mol,Belgium

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Other culture collection numbers:CGSC 5461

　　Medium:LB-Lennox

　　Selection marker:/

　　Temperature:37°C

　　Genotype:Hfr gal-55λ-relA1 spoT1 thiE1 hsdR4(Source:description CGSC 5461)

　　Phenotype:r-mK+

　　Properties:-

　　Further information:The strain number in the SCK collection is CM0191.

　　Original reference:Boyer and Roulland-Dussoix,J.Mol.Biol.41(1969),459-472[PMID:4896022]

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli K12 HB94(LMBP 7992)is available at BCCM/GeneCorner.It was deposited by M.Mergeay and A.Provoost and was published in Boyer and Roulland-Dussoix,1969.