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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:WK6mutS(λ)

　　Accession number:LMBP 1466

　　Depositor:Prof.Dr E.Remaut1 2

　　1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium

　　2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Lennox

　　Selection marker:/

　　Temperature:28°C(Note:37°C is also possible but phage lambda remains more stably integrated when grown at 28°C)

　　Genotype:F'lacIqΔ(lacZ)M15 proA+B+traD36/Δ(lac-proB)galE rpsL mutS::Tn10λ

　　Phenotype:StrR TetR

　　Properties:Useful strain for site-directed mutagenesis;disabled mismatch repair due to knocked-out mutS.The Tn10 insertion renders the strain tetR.

　　Further information:Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations.

　　Original reference:Zell et al.,EMBO J.6(1987),1809-1815[PMID:3038536]

　　Related reference:Stanssens et al.,Nucleic Acids Res.17(1989),4441-4454[PMID:2501754]

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli K12 WK6mutS(λ)(LMBP 1466)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Zell et al.,1987.