Species:Escherichia coli
Group:K12
Strain designation:WK6mutS(λ)
Accession number:LMBP 1466
Depositor:Prof.Dr E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:/
Temperature:28°C(Note:37°C is also possible but phage lambda remains more stably integrated when grown at 28°C)
Genotype:F'lacIqΔ(lacZ)M15 proA+B+traD36/Δ(lac-proB)galE rpsL mutS::Tn10λ
Phenotype:StrR TetR
Properties:Useful strain for site-directed mutagenesis;disabled mismatch repair due to knocked-out mutS.The Tn10 insertion renders the strain tetR.
Further information:Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations.
Original reference:Zell et al.,EMBO J.6(1987),1809-1815[PMID:3038536]
Related reference:Stanssens et al.,Nucleic Acids Res.17(1989),4441-4454[PMID:2501754]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 WK6mutS(λ)(LMBP 1466)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Zell et al.,1987.
Group:K12
Strain designation:WK6mutS(λ)
Accession number:LMBP 1466
Depositor:Prof.Dr E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:/
Temperature:28°C(Note:37°C is also possible but phage lambda remains more stably integrated when grown at 28°C)
Genotype:F'lacIqΔ(lacZ)M15 proA+B+traD36/Δ(lac-proB)galE rpsL mutS::Tn10λ
Phenotype:StrR TetR
Properties:Useful strain for site-directed mutagenesis;disabled mismatch repair due to knocked-out mutS.The Tn10 insertion renders the strain tetR.
Further information:Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations.
Original reference:Zell et al.,EMBO J.6(1987),1809-1815[PMID:3038536]
Related reference:Stanssens et al.,Nucleic Acids Res.17(1989),4441-4454[PMID:2501754]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 WK6mutS(λ)(LMBP 1466)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Zell et al.,1987.
