Species:Escherichia coli
Group:K12
Strain designation:WG914
Accession number:LMBP 12434
Depositor:This host strain was deposited by Dr D.E.Culham1 and Prof.Em.Dr J.M.Wood1.
1 Department of Molecular and Cellular Biology,University of Guelph,Guelph,Canada
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:-
Temperature:37°C
Genotype:F-trp lacZ rpsL thi?putPA101 proU205?proQ676
Phenotype:StrR
Properties:-
Further information:This host strain was created by inserting an in-frame chromosomal proQ deletion(?proQ676)into E.coli K12 WG210.
Resistance to streptomycin has been experimentally confirmed by BCCM/GeneCorner.
Original reference:Smith et al.,Biochemistry 43(2004),12979-12989[PMID:15476391][DOI:10.1021/bi048561g]
Restricted use:-BCCM MTA
-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Refer in your Materials and Methods:
Escherichia coli K12 WG914(LMBP 12434)is available at BCCM/GeneCorner.It was deposited by This host strain was deposited by Dr D.E.Culham and Prof.Em.Dr J.M.Wood and was published in Smith et al.,2004.
Group:K12
Strain designation:WG914
Accession number:LMBP 12434
Depositor:This host strain was deposited by Dr D.E.Culham1 and Prof.Em.Dr J.M.Wood1.
1 Department of Molecular and Cellular Biology,University of Guelph,Guelph,Canada
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:-
Temperature:37°C
Genotype:F-trp lacZ rpsL thi?putPA101 proU205?proQ676
Phenotype:StrR
Properties:-
Further information:This host strain was created by inserting an in-frame chromosomal proQ deletion(?proQ676)into E.coli K12 WG210.
Resistance to streptomycin has been experimentally confirmed by BCCM/GeneCorner.
Original reference:Smith et al.,Biochemistry 43(2004),12979-12989[PMID:15476391][DOI:10.1021/bi048561g]
Restricted use:-BCCM MTA
-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Refer in your Materials and Methods:
Escherichia coli K12 WG914(LMBP 12434)is available at BCCM/GeneCorner.It was deposited by This host strain was deposited by Dr D.E.Culham and Prof.Em.Dr J.M.Wood and was published in Smith et al.,2004.
