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　　Species:Lactococcus lactis

　　Group:

　　Strain designation:subsp.cremoris MG1363

　　Accession number:LMBP 3019

　　Depositor:Prof.Dr E.Remaut1 2

　　1 VIB-UGent Center for Inflammation Research,VIB,Ghent,Belgium

　　2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium

　　It was obtained from Dr J.Wells3

　　3 Microbiol Genetics and Physiology Section,Institute of Food Research,Colney,Norwich,UK

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:M17+0.5%glucose(GM17)

　　Selection marker:/

　　Temperature:28°C

　　Cultivation remarks:Cultivate under unaerated conditions for at least 20 hours.

　　Genotype:-

　　Phenotype:-

　　Properties:MG1363 is a derivative of L.lactis subsp.cremoris NCDO 712,which was cured of all resident plasmids.Consequently,it cannot ferment lactose and does not produce extracellular protease.

　　Further information:The full genome sequence of this strain is available at the EMBL Nucleotide Sequence Database under accession number AM406671.1.

　　Original reference:Gasson,J.Bacteriol.154(1983),1-9[PMID:6403500]

　　Restricted use:-BCCM MTA

　　Cultivation remarks:Cultivate under unaerated conditions for at least 20 hours.

　　Refer in your Materials and Methods:

　　Lactococcus lactis subsp.cremoris MG1363(LMBP 3019)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Gasson,1983.