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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:WG389

　　Accession number:LMBP 12441

　　Depositor:This host strain was deposited by Dr D.E.Culham1 and Prof.Em.Dr J.M.Wood1.

　　1 Department of Molecular and Cellular Biology,University of Guelph,Guelph,Canada

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Lennox+pro(25 mM)

　　Selection marker:-

　　Temperature:37°C

　　Genotype:F-trp lacZ rpsL thi?putPA101?(proU)600?(proP-melAB)212?(brnQ phoA proC)

　　Phenotype:StrR

　　Properties:This is a proline auxotroph lacking all known proline uptake systems.

　　Further information:Resistance to streptomycin has been experimentally confirmed by BCCM/GeneCorner.

　　Original reference:Culham et al.,J.Mol.Biol.229(1993),268-276[PMID:8421314][DOI:10.1006/jmbi.1993.1030]

　　Restricted use:-BCCM MTA

　　-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

　　Refer in your Materials and Methods:

　　Escherichia coli K12 WG389(LMBP 12441)is available at BCCM/GeneCorner.It was deposited by This host strain was deposited by Dr D.E.Culham and Prof.Em.Dr J.M.Wood and was published in Culham et al.,1993.