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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:MG1655 SEGA025

　　Accession number:LMBP 12861

　　Depositor:A.Ehrmann1 and Prof.Dr M.N?rholm1.It was constructed by C.Bayer1,A.Ehrmann1 and M.Rennig1.

　　1 The Novo Nordisk Foundation Center for Biosustainability,Technical University of Denmark,Horsholm,Denmark

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Other culture collection numbers:SEGA 025

　　Medium:LB-Lennox

　　Selection marker:tetracycline(10μg/ml)

　　Temperature:37°C

　　Genotype:F-λ-ilvG-rfb-50 rph-1 glmS-pstS::Ptrc-high-Ubiquitin-gfp-tetA(Source:http://openwetware.org/wiki/E._coli_genotypes#MG1655+Bayer et al.,2021)

　　SEGA sequence:p12861.gb

　　Phenotype:tetR

　　Properties:The strain was created based on Escherichia coli K12 MG1655,by integrating the SEGA landing pad between the glmS and pstS loci.Counter-selection is posssible through sensitivity to NiCl2(50μM).

　　Further information:This host strain is intended for simplistic bacterial genome engineering,using the SEGA genome architecture.

　　Original reference:Bayer et al.,Nat.Commun.12(2021),5876[DOI:10.1038/s41467-021-26155-5]

　　Restricted use:-BCCM MTA

　　-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

　　Refer in your Materials and Methods:

　　Escherichia coli K12 MG1655 SEGA025(LMBP 12861)is available at BCCM/GeneCorner.It was deposited by A.Ehrmann and Prof.Dr M.N?rholm and was published in Bayer et al.,2021.