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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:MG1655 seqA-PAmCherry

　　Accession number:LMBP 9778

　　Depositor:Prof.Dr J.Michiels1.It was constructed by T.Swings1

　　1 Centre of Microbial and Plant Genetics,Department of Microbial and Molecular Systems,KU Leuven,Leuven,Belgium

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Miller

　　Selection marker:kanamycin(50μg/ml)

　　Temperature:37°C

　　Genotype:F-λ-ilvG-rfb-50 rph-1 seqA-PAmCherry:km(R)(Source:http://openwetware.org/wiki/E._coli_genotypes#MG1655+Mika et al.,2015)

　　Phenotype:KanR

　　Properties:This strain can be used to study the subcellular localization of the E.coli DNA binding protein SeqA.

　　Further information:The native seqA gene was fused at its 3'end to the fluorescent protein gene PAmCherry,separated with a linker(ASPPPGRSR)and followed by a kanamycin resistance marker.The entire cassette was integrated in the chromosome.

　　PAmCherry is a photoactivatable variant of the red fluorescent protein mCherry.PAmCherry is not fluorescent until it is exposed to 350-400 nm light.

　　Original reference:Mika et al.,Faraday Discuss.184(2015),425-450[PMID:26449690][DOI:10.1039/c5fd00058k]

　　Restricted use:-BCCM MTA

　　-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded,namely UGent-DBMB and VIB-IRC.

　　Refer in your Materials and Methods:

　　Escherichia coli K12 MG1655 seqA-PAmCherry(LMBP 9778)is available at BCCM/GeneCorner.It was deposited by Prof.Dr J.Michiels and was published in Mika et al.,2015.