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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:SM10(λpir)

　　Accession number:LMBP 3889

　　Depositor:Dr J.J.Mekalanos1

　　1 Department of Microbiology and Molecular Genetics,Harvard Medical School,Boston,USA

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Lennox

　　Selection marker:kanamycin(50μg/ml)

　　Temperature:37°C

　　Genotype:thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmλpir

　　Phenotype:KanR

　　Properties:Donor strain(mobilizing strain)carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome.Useful for mobilizing mobRP4 plasmids.

　　Further information:The presence ofλpir allows the replication of a defective R6K suicide plasmid and derivatives thereof.

　　Original reference:-

　　Related reference:Simon et al.,Biotechnology 1(1983),784-791

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli K12 SM10(λpir)(LMBP 3889)is available at BCCM/GeneCorner.It was deposited by Dr J.J.Mekalanos.