Species:Escherichia coli
Group:K12
Strain designation:SG4121
Accession number:LMBP 671
Depositor:Prof.E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
It was obtained from Dr S.Gottesman3
3 Laboratory of Molecular Biology,NCI,NIH,Bethesda,USA
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:lon-100Δ(gal-pgl)324 lac thi rpsL recA hsdR
Phenotype:StrR Lon-rK-mK+non-mucoid
Properties:-
Further information:The Lon protease plays a major role in the proteolytic machinery of E.coli.Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle.The(gal-pgl)deletion eliminates mucoidy and restores normal colony morphology.
Lon mutants may be useful in stabilizing heterologous proteins expressed in E.coli(see e.g.Remaut et al.,1983).
In addition,this strain is K12 restriction negative/modification positive,and RecA deficient.
Original reference:-
Related reference:Remaut et al.,Nucleic Acids Res.11(1983),4677-4688[PMID:6308555]
Keidel et al.,Eur.J.Biochem.204(1992),1141-1148[PMID:1312934]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 SG4121(LMBP 671)is available at BCCM/GeneCorner.It was deposited by Prof.E.Remaut.
