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SG4044
    SG4044
  • 平臺(tái)編號(hào):bio-127942
  • 國(guó)際編號(hào):LMBP 670
  • 拉丁屬名: Escherichia coli
  • 規(guī)格:/
  • 用途:BCCM原裝進(jìn)口
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購(gòu) 說(shuō)明書(shū)
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

  Species:Escherichia coli

  Group:K12

  Strain designation:SG4044

  Accession number:LMBP 670

  Depositor:Prof.E.Remaut1 2

  1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium

  2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium

  It was obtained from Dr S.Gottesman3

  3 Laboratory of Molecular Biology,NCI,NIH,Bethesda,USA

  Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

  Medium:LB-Lennox

  Selection marker:/

  Temperature:37°C

  Genotype:lon-100Δ(gal-pgl)324 lac thi rpsL

  Phenotype:StrR Lon-non-mucoid

  Properties:-

  Further information:The Lon protease plays a major role in the proteolytic machinery of E.coli.Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle.The(gal-pgl)deletion eliminates mucoidy and restores normal colony morphology.

  Lon mutants may be useful in stabilizing heterologous proteins expressed in E.coli(see e.g.Remaut et al.,1983).

  Original reference:Gottesman et al.,J.Bacteriol.148(1981),265-273[PMID:7026534]

  Related reference:Remaut et al.,Nucleic Acids Res.11(1983),4677-4688[PMID:6308555]

  Gottesman et al.,J.Bacteriol.162(1985),1111-1119[PMID:3888955]

  Restricted use:-BCCM MTA

  Refer in your Materials and Methods:

  Escherichia coli K12 SG4044(LMBP 670)is available at BCCM/GeneCorner.It was deposited by Prof.E.Remaut and was published in Gottesman et al.,1981.

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