Species:Escherichia coli
Group:K12
Strain designation:S26R1d
Accession number:LMBP 4979
Depositor:Prof.Dr E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
It was obtained from Dr A.Garen3
3 Department of Molecular Biology and Biophysics,Yale University,USA
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Other culture collection numbers:CGSC 4617
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:Hfr fhuA22 phoA4(Am)glnV33(AS)ompF627(T2R)fadL701(T2R)relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510(Source:CGSC 4617)
Phenotype:phosphatase positive
Properties:This strain carries the S26 amber mutation(TAG)in the phosphatase gene.This strain carries the supE suppressor(glnV33(AS):inserts glutamine at the amber position)and is therefore capable of suppressing the S26 phosphatase nonsense mutation,as well as amber mutations in other genes.However,the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.
Further information:-
Original reference:Garen et al.,J.Mol.Biol.14(1965),167-178[PMID:5327650]
Related reference:Remaut et al.,J.Mol.Biol.71(1972),243-261[PMID:4564480]
Weigert et al.,J.Mol.Biol.14(1965),522-527[PMID:4286570]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 S26R1d(LMBP 4979)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Garen et al.,1965.
Group:K12
Strain designation:S26R1d
Accession number:LMBP 4979
Depositor:Prof.Dr E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
It was obtained from Dr A.Garen3
3 Department of Molecular Biology and Biophysics,Yale University,USA
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Other culture collection numbers:CGSC 4617
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:Hfr fhuA22 phoA4(Am)glnV33(AS)ompF627(T2R)fadL701(T2R)relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510(Source:CGSC 4617)
Phenotype:phosphatase positive
Properties:This strain carries the S26 amber mutation(TAG)in the phosphatase gene.This strain carries the supE suppressor(glnV33(AS):inserts glutamine at the amber position)and is therefore capable of suppressing the S26 phosphatase nonsense mutation,as well as amber mutations in other genes.However,the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.
Further information:-
Original reference:Garen et al.,J.Mol.Biol.14(1965),167-178[PMID:5327650]
Related reference:Remaut et al.,J.Mol.Biol.71(1972),243-261[PMID:4564480]
Weigert et al.,J.Mol.Biol.14(1965),522-527[PMID:4286570]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 S26R1d(LMBP 4979)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Garen et al.,1965.
