Species:Escherichia coli
Group:K12
Strain designation:S26
Accession number:LMBP 4977
Depositor:Prof.Dr E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
It was obtained from Dr A.Garen3
3 Department of Molecular Biology and Biophysics,Yale University,USA
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Other culture collection numbers:CGSC 4620
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:Hfr garB10 fhuA22 phoA4(Am)ompF627(T2R)fadL701(T2R)relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510(Source:CGSC 4620)
Phenotype:phosphatase negative
Properties:This strain carries the S26 amber mutation(TAG)in the phosphatase gene.This strain is the parental strain of a series of four isogenic strains differing only in their suppressor activity(see accession numbers LMBP 4977 up to and including LMBP 4980).
Further information:-
Original reference:Garen et al.,J.Mol.Biol.14(1965),167-178[PMID:5327650]
Related reference:Remaut et al.,J.Mol.Biol.71(1972),243-261[PMID:4564480]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 S26(LMBP 4977)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Garen et al.,1965.
Group:K12
Strain designation:S26
Accession number:LMBP 4977
Depositor:Prof.Dr E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
It was obtained from Dr A.Garen3
3 Department of Molecular Biology and Biophysics,Yale University,USA
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Other culture collection numbers:CGSC 4620
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:Hfr garB10 fhuA22 phoA4(Am)ompF627(T2R)fadL701(T2R)relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510(Source:CGSC 4620)
Phenotype:phosphatase negative
Properties:This strain carries the S26 amber mutation(TAG)in the phosphatase gene.This strain is the parental strain of a series of four isogenic strains differing only in their suppressor activity(see accession numbers LMBP 4977 up to and including LMBP 4980).
Further information:-
Original reference:Garen et al.,J.Mol.Biol.14(1965),167-178[PMID:5327650]
Related reference:Remaut et al.,J.Mol.Biol.71(1972),243-261[PMID:4564480]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 S26(LMBP 4977)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Garen et al.,1965.
