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平臺編號：bio-136765
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國際編號：hkb-htlr5
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拉丁屬名： Human TLR5 Reporter HEK293 Cells (NF-κB)
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規(guī)格：3-7 x 10e6 cells
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用途：invivnogen原裝
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Human TLR5 Reporter HEK293 Cells (NF-κB)
NF-κB–SEAP reporter HEK293 cells expressing human TLR5
HEK-Blue?-hTLR5 cells were obtained by co-transfection of the human TLR5 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR5 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue? Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue? hTLR5 cells is HEK-Blue? Null1 cells.
HEK-Blue?-hTLR5 cells were obtained by co-transfection of the human TLR5 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR5 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue? Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue? hTLR5 cells is HEK-Blue? Null1 cells.
