Limosilactobacillus fermentum (Beijerinck) Zheng et al.
ATCC 8289
Product category
Bacteria
Classification
Lactobacillaceae
Strain designation
[NCDO 218, NCIB 7230, NCTC 7230]
Type strain
No
Former name
Lactobacillus fermentum Beijerinck
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Preceptrol
No
Handling information
Medium
ATCC Medium 416: Lactobacilli MRS Agar/Broth
Temperature
37°C
Handling procedure
Open vial.
From a single tube of #416 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
Use 0.1 ml of this suspension to inoculate #416 slants and 0.1ml to inoculate #416 plates.
Incubate tubes and plates at 37°C, under aerobic conditions, for 48 hours.
After 48 hours of incubation, wash cells from the slant and transfer this broth to a new slant and plate. Incubate another 48 hours under aerobic conditions. This second transfer and incubation is necessary for complete removal of the cryoprotectant, which can inhibit growth.
Handling notes
Additional information on this culture is available on the ATCC web site at www.atcc.org.
History
Deposited as
Lactobacillus fermentum Beijerinck
Depositors
RH Vaughn
Chain of custody
ATCC <-- RH Vaughn <-- C.S. Pederson (Lactobacillus gayonii)
