Name:
Caniella muris
DSM No.:
110323, Proposed type strain
Strain designation:
CLA-SR-94
Isolated from:
caecal content; wild mouse
Country:
Germany
Aachen
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- T. Clavel, RWTH Aachen; CLA-SR-94
Genbank accession numbers:
16S rRNA gene: OM658553
Cultivation conditions:
Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media 5% horse blood., 37°C
For reactivation use the complete content of the ampoule for inoculation.
Please follow special instructions: 'Cultivation of Anaerobes'
Complete DSMZ Media List
Summary and
additional information:
<- T. Clavel, RWTH Aachen; CLA-SR-94. Caecal content; wild mouse. Germany, Aachen. Sequence accession no. 16S rRNA gene: OM658553.poposed name: Caniella muris
. (Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media 5% horse blood., incubation time: 2-3 days, gas phase: 80%N2, 20%CO2, pH 7, 37°C, anaerobic). For reactivation use the complete content of the ampoule for inoculation.
Risk group:
2 (provisional classification by DSMZ)
