ATCC Medium: 44 Brain Heart Infusion Agar/Broth
Agar Medium
Brain Heart Infusion Agar (BD 211065)…………………..52 g
DI Water……………………...………………………………1000 ml
Autoclave at 121?C.
Broth Medium
Brain Heart Infusion Broth (BD 237500)…………..…….37.0g
DI Water…………………………………………………….1000 ml
Autoclave at 121?C.
*This product is commercially available from BD.
To make medium from scratch, follow formulation below:
Brain Heart Infusion Composition
Calf Brains, infusion from…………………………………..200 g
Beef Hearts, infusion from………………………………….250 g
Proteose Peptone…………………………………...………10.0 g
Dextrose……………………………………………..………..2.0 g
NaCl…………………………………………………...………5.0 g
Na2HPO4…………………………………………...………...2.5 g
DI Water……………………………………………………….1000 ml
Final pH 7.4 +/- 0.2
*Omit agar for broth medium.
Final Product Description ?
Medium should be tan in color. ?
Recommended storage temperature: 2-8?C.