ATCC Medium: 1395 Middlebrook 7H9 Broth w/ADC Enrichment
Middlebrook 7H9 Broth………………………………4.7g
Glycerol………………………………………………..2.0 ml
DI Water……...........................................................900 ml
Mix well. Autoclave at 121?C for 10 minutes.
Aseptically add 100ml
ADC Enrichment:
ADC Enrichment Bovine Serum Albumin, Frac V……………………..5.0 g
Dextrose……………………………………………….2.0 g
Catalase (beef)………………………………………..3 mg
DI Water……………………………………………….100ml
Filter Sterilize.
Store any unused portion with lot information and Media Number in 2-8?C.