ATCC Medium: 336 Potato Dextrose Agar (PDA)
Potato Dextrose Agar (BD 213400)………………………..39.0 g
DI Water……………………………………………………….1000 ml
Autoclave at 121?C.
*This product is commercially available from BD.
To make medium from scratch, follow formulation below:
Potato Dextrose Agar Composition
Diced potatoes................................300.0 g
Glucose...........................................20.0 g
Agar................................................15.0 g
DI Water…………………………….1000 ml
Boil finely diced potatoes in 500 ml of water until thoroughly cooked (about 1 hour); filter through cheesecloth and add water to filtrate to 1000 ml. Heat the filtrate to dissolve the agar. Add the glucose before sterilization. Autoclave at 121?C.