ATCC medium: 1553 Alginate utilization medium
Solution A:
NaCl....................................27.5 g
MgSO4 . 6H2O .............................6.78 g
MgCl2 . 6H2O .............................5.38 g
CaCl2 . 2H2O .............................1.4 g
KCl......................................0.72 g
NaHCO3 ...................................0.2 g
NaNO3 ...................................47.0 mg
Tris HCl................................67.0 mg
alpha-Sodium glycerophosphate............6.67 mg
Distilled water........................500.0 ml
Autoclave Solution A at 121C for 15 minutes. Cool to 50C and aseptically add Solutions C and D. Then Aseptically add this solution to Solution B. For liquid medium omit agar, reduce sodium alginate to 1.0 g/L, calcium chloride to 0.14 g/L, magnesium chloride to 0.538 g/L and magnesium sulfate to 0.678 g/L.
Solution B:
Sodium alginate.........................10.0 g
Agar (if needed)........................20.0 g
Distilled water........................480.0 ml
Solution C: Wolfe's Mineral Solution (see below)....10.0 ml
Filter-sterilize. Solution D: Vitamin B12..............................1.3 mcg
Thiamine . HCl..........................67.0 mcg Biotin...................................0.67 mcg
Distilled water.........................10.0 ml
Filter-sterilize.