Sol A
Tryptone …………………………..10.0 g
Yeast Extract………………………1.0 g
NaCl…………………………………8.0 g
Agar (if required)…………………15.0 g
DI Water…………………………..950 mL
Autoclave at 121?C Aseptically add the following:
Sol B
Glucose……………………1.0 g
CaCl2……………………….0.294 g
Thiamine…………………..10.0 mg
DI water …………………..50 mL
Aseptically dispense into appropriate vessels