NaCl...................................250.0 g
Casamino Acids (BD 223050)...............7.5 g
Proteose Peptone No. 3 (BD 211693).......5.0 g
Yeast extract............................1.0 g
Sodium citrate...........................3.0 g
MgSO4 . 7H2O ............................20.0 g
K2HPO4 ...................................0.5 g
Fe(NH4)2(SO4)2 . 6H2O .....................0.05 g
Distilled water..........................1.0 L
Adjust pH to 7.0 with KOH.
Autoclave at 121C for 15 minutes.