ATCC Medium: 2790 Artificial Mono Lake Medium w/ Arsenate
(NH4)2SO4.................................................................... 0.1g
MgSO4.7H2O………………………………………………0.025g
NaCl……………………………………………………….60g
Na2CO3…………………………………………………….10.6g
NaHCO3……………………………………………………4.2 g
Na2AsHN2O4 x 7H2O……………..……………………….6.24 g
SL10 Trace Elements (see below)………………………5 ml
Glucose solution (add aseptically; see below)…………50 ml
Wolfe’s Vitamin (add aseptically)…………………….…10 ml
DI Water……………………………………………………940 ml
Mix all ingredients except for vitamins and glucose solution. Adjust pH to 9.85 with NaOH. Autoclave at 121?C and let cool. Aseptically add vitamins and glucose solution. Dispense into aerobic culture vessels as desired.
HCl (25%)…………………………………………………..10.0 ml
SL10 Trace Elements
FeCl2 x 4H2O………………………………………………1.50 g
ZnCl2………………………………………………………..70.0 mg
MnCl2 x 4H2O………………………………………………100.0 mg
H3BO3……………………………………………………….6.0 mg
CoCl2 x 6H2O………………………………………………190.0 mg
CuCl2 x 2H2O………………………………………………2.0 mg
NiCl2 x 6H2O……………………………………………….24.0 mg
Na2MoO4 x 2H2O…………………………………………36.0 mg
Na2WO4 x 2H2O………………………………………….3.0 mg
DI Water…………………………………………………….990.0 ml
Glucose……………………………………………………1.8 g
Glucose Solution
DI Water……………………………………………………50 ml