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ATCC medium: 2453 DH medium

中國(guó)微生物菌種查詢網(wǎng) / 2016-11-15 09:11:36

ATCC medium: 2453 DH medium

Complete medium:

Medium D (20X) (see below)............. 50.0 ml

HEPES....................................1.2 g

Distilled deionized water................1.0 L

Adjust pH to 8.24-8.26 then autoclave at 121C. Leave to cool in dark cupboard overnight. The next day test the pH to make sure it is appropriate. When first prepared and autoclaved, Medium D may be slightly cloudy or turbid. It should clear as it cools and remain clear in storage. The medium should be stored in the dark for 1- 2 days before using for culture work.

Medium D--20X Concentration:

NTA(nitrilotriacetic acid)...............2.0 g

Micronutrient solution (see below)......10.0 ml

FeCl3 solution (0.29 g/liter) ..........20.0 ml

CaSO4 . 2H2O .............................1.2 g

MgSO4 . 7H2O .............................2.0 g

NaCl.....................................0.16 g

KNO3 .....................................2.0 g

NaNO3 ...................................14.0 g

Na2HPO4 ..................................2.2 g

Distilled deionized water................1.0 L

Filter-sterilize to store for longer than one week.

Micronutrient Solution:

H2SO4 (concentrated) .....................0.5 ml

MnSO4 . H2O ..............................2.28 g

ZnSO4 . 7H2O .............................0.50 g

H3BO3 ....................................0.50 g

CuSO4 . 5H2O .............................0.025 g

Na2MoO4 . 2H2O............................0.025 g

CoCl2 . 6H2O .............................0.045 g

Distilled deionized water................1.0 L

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