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ATCC medium: 1047 MN Marine medium (ATCC Medium 957) with 20

中國微生物菌種查詢網(wǎng) / 2016-11-15 09:29:44

ATCC medium: 1047 MN Marine medium (ATCC Medium 957) with 20 mcg/L of vitamin B12 ATCC Medium 957 (see below) with 20 mcg/L vitamin B12 added

ATCC Medium 957:

MgSO4 . 7H2O .......................... 0.04 g

CaCl2 . 2H2O .......................... 0.02 g

NaNO3 ................................. 0.75 g

K2HPO4 . 3H2O.......................... 0.02 g

Citric acid........................... 3.0 mg

Ferric ammonium citrate............... 3.0 mg

DTA.................................. 0.5 mg

Na2CO3 ................................ 0.02 g

Trace Metals A-5 (see below).......... 1.0 ml

Agar Noble (Difco 0142) (if needed).. 10.0 g

Distilled water..................... 250.0 ml

Seawater............................ 750.0 ml

Adjust pH after autoclaving to 8.5 with KOH.

Trace Metals A-5:

H3BO3 .......................... 2.86 g

MnCl2 . 4H2O ................... 1.81 g

ZnSO4 . 7H2O ................... 0.222 g

Na2MoO4 . 2H2O ................. 0.039 g

CuSO4 . 5H2O ................... 0.079 g

Co(NO3)2 . 6H2O................ 49.4 mg

Distilled water................ 1.0 L

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