Solution A (see below)..............................................0.5 ml
Solution B (see below)..............................................0.5 ml
Solution C (see below).............................................1.0 ml
Solution D (see below).............................................0.5 ml
Solution E (see below).............................................0.5 ml
Solution F (see below).............................................2.0
drops DI Water to..............................................................1000 ml
Autoclave at 121?C.
Solution A
CaCl2 ...........................................................2.0 g
DI Water……………………………………….100.0 ml
Solution B
MgSO4 . 7H2O……………………................20.0 g
DI Water………………………………............100.0 ml
Solution C
EDTA.............................................................0.14 g
FeSO4 . 7H2O……………………..................0.5 g
H2SO4 (conc.)……………………..................0.05 ml
DI Water……………………………….............100.0 ml
Solution D
(trace metals) Na2MoO4 . 2H2O..........................................0.1 g
MnCl2 . 4H2O……………………...................0.2 g
CoCl2 . 6H2O……………………...................0.002 g
ZnSO4 . 7H2O……………………..................0.1 g
CuSO4 . 5H2O……………………..................0.02 g
DI Water………………………………….…….1000 ml
These ingredients should be dissolved separately and then added together to make 1000 ml.
Solution E
NaNO2 ..........................................................41.4 g
DI Water……………………………….............100.0 ml
Solution F
K2HPO4………………………........................1.74 g
DI Water……………………………….............100.0 ml