ATCC Medium: 2546 Mineral Medium pH 7.25
Base Medium
KH2PO4……………………………………….…0.41 g
Na2HPO4………………………………………...0.53 g
NaClO3…………………………………………..1.06 g
CaCl2……………………………………………..0.11 g
MgCl2…………………………………….............0.1 g
NH4HCO3………………………………………..0.44 g
NaHCO3………………………………….............3.73 g
Na2S . 9H2O………………………………………..0.5 g
Resazurin (0.5g/liter)……………………………..1.00 ml
Acid Trace elements (see below)………………....1.00 ml
Alkaline Trace elements (see below)…………….1.00 ml
*ATCC vitamins…………………………………..1.00 ml
DI Water…………………………………………..1000 ml
Boil under 100% N2
Distribute into appropriate vessel, seal under 100% N2 and autoclave at 121C.
*Filter sterilize ATCC vitamins and add to autoclaved medium
. Acid trace elements
FeCl2 . 4H2O……………….1.485 g
H3BO3……………………0.062
ZnCl2……………………..0.068
CaCl2……………………..0.013
MnCl2 . 4H2O……………...0.098
CaCl2 . 6H2O………………0.119
NiCl2 . 6H2O………………0.119
HCl….4.100 ml
Alkaline trace elements
N2SeO3 . 5H2O …………….0.026 g
Na2WO4 . 2H2O …………….0.033
Na2MoO4 . 2H2O…………...0.024
NaOH……………………..0.400