MgSO4 . 7H2O .............................1.0 g
CaCl2 . 6H2O .............................0.20 g
Chelated Iron Solution (see below).......2.0 ml
KNO3 .....................................1.0 g
Trace Element Solution (see below).......0.5 ml
KH2PO4 ...................................0.272 g
Na HPO . 12H O...........................0.717 g
2 4 2 Purified Agar (e.g., Oxoid L28).........12.5 g
Distilled deionized water................1.0 L
Adjust pH to 6.8. Autoclave at 121C for 15 minutes.
Chelated Iron Solution:
Ferric (III) ammonium citrate*...........0.1 g
EDTA, sodium salt........................0.2 g
HCl (concentrated).......................0.3 ml
Distilled deionized water..............100.0 ml
*0.05 g of Ferric (III) chloride may be substituted.Use 2.0 ml of this chelated iron solution per liter of final medium.
Trace Element Solution:
EDTA.................500.0 mg
FeSO4 . 7H2O .........200.0 mg
ZnSO4 . 7H2O ..........10.0 mg
MnCl2 . 4H2O ...........3.0 mg
H3 3 BO .................30.0 mg
CoCl2 . 6H2O ..........20.0 mg
CaCl2 . 2H2O ...........1.0 mg
NiCl . 6H O ...........2.0 mg
2 2 Na2MoO4 . 2H2O..........3.0 mg
Distilled water........1.0 L
Autoclave at 121C for 15 minutes.