ATCC medium: 724 CML medium
Cooked Meat (Oxoid CM 82)...............57.0 g
Liver Infusion Broth (see below)........10.0 ml
Glucose.................................10.0 g
Tryptose (BD 211713)....................10.0 g
Distilled water to.......................1.0 L
Dissolve cooked meat in distilled water and place at 4C to clear. Filter through cheesecloth before adding the other ingredients. Autoclave complete medium at 121C for 15 minutes.
Liver Infusion Broth: Prepare this infusion from Liver (BD 213320) as directed on the label.