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ATCC medium: 2644 Telmatospirillum Medium

中國微生物菌種查詢網(wǎng) / 2016-11-16 14:14:48

ATCC medium: 2644 Telmatospirillum Medium

Na pyruvate..............................3.00 g

K2HPO4 ...................................0.25 g

KH2PO4 ...................................1.00 g

(NH4)2SO4................................1.00 g

CaCl2 . 2H2O .............................0.10 g

MgSO4....................................0.40 g

Mineral solution (see below)............10.00 ml

Distilled water..........................1.00 L

Prepare the medium anaerobically under a steady stream of 80% N2 and 20% CO2. Adjust the pH to 5.5-6.5 with 1M H3PO4. Autoclave at 121?C for 15 minutes.

Mineral Solution:

Nitrilotriacetic acid .......1.500 g

FeCl.........................0.100 g

KI...........................0.020 g

CoCl . 6H20..................0.020 g

MnCl2 . 4H2O .................0.080 g

ZnSO4 . 7H O .................0.080 g 2

H3BO3 ........................0.010 g

Na2MoO4 . 2H2O................0.010 g

CuCl2 ........................0.010 g

NiCl2 . 6H2O .................0.020 g

Distilled water..............1.000 L

Add nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve the compound. Bring volume to 1.0 L with remaining water and add remaining compounds one at a time.

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