Solution A
Mueller-Hinton Broth……………………...22.0 g
Yeast Extract………………………………5.0 g
DI Water……………………………………900 ml
Autoclave at 121C.
Solution B
Hemin (dissolve in NaOH)………………..15.0 mg
Nicotinamide adenine dinucleotide……...15.0 mg
DI Water…………………………………….100 ml
Filter sterilize.
Complete medium: Add solutions A and B together. Adjust for final pH 7.3 +/- 0.1. Dispense as required