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ATCC medium: 2061 PYE Medium

中國(guó)微生物菌種查詢網(wǎng) / 2016-11-22 14:24:57

K2HPO4 ...................................1.0 g

MgSO4 . 7H2O .............................0.2 g

CaCl2 . 2H2O ............................20.0 mg

Na2S2O3 . 5H2O ............................0.2 g

Sodium pyruvate..........................2.2 g

Yeast extract............................4.0 g

(NH4)2SO4.................................1.0 g

Trace Elements Solution(see below)......1.0 ml

Chelated Iron Solution (see below).......2.0 ml

d-Biotin................................15.0 mcg

Vitamin B12.............................20.0 mcg

Distilled water..........................1.0 L

Adjust medium for final pH 6.9. Autoclave at 121C for 15 minutes.

Trace Elements Solution:

EDTA.........................2.5 mg

MnCl2 . 4H2O .................0.2 g

H3BO3 ........................0.1 g

Na2MoO4 . 2H2O................0.1 g

ZnCl2 .......................50.0 mg

NiCl2 . 6H2O ................50.0 mg

CoCl2 . 6H2O ................20.0 mg

CuCl2 . 2H2O .................5.0 mg

VOSO4 . 2H2O .................5.0 mg

Na2WO4 . 2H2O.................5.0 mg

Distilled water............250.0 ml

Chelated Iron Solution:

EDTA.........................1.0 g

FeCl3 . 6H2O .................0.5 g

Concentrated HCl.............1.5 ml

Distilled water..............1.0 L

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