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ATCC medium: 461 Castenholz TYE medium

中國(guó)微生物菌種查詢網(wǎng) / 2016-11-23 10:34:46

Mix aseptically 5 parts double strength Castenholz Salts (see below) with one part 1% TYE (see below) and 4 parts distilled water.

Final pH of complete medium should be 7.6.

Castenholz Salts, 2X:

Nitrilotriacetic acid....................0.2 g

Nitsch's Trace Elements (see below)......2.0 ml

FeCl3 solution (0.03%) ...................2.0 ml

CaSO4 . 2H2O .............................0.12 g

MgSO . H O ..............................0.2 g

4 2 NaCl.....................................0.016 g

KNO3 .....................................0.21 g

NaNO ....................................1.4 g

3 Na2HPO4 ..................................0.22 g

Agar (if needed)........................30.0 g

Distilled water..........................1.0 L

Adjust pH to 8.2.

Nitsch's Trace Elements:

H2SO4 ..............................0.5 ml

MnSO4 ..............................2.2 g

ZnSO4 . 7H2O........................0.5 g

H3BO3 ..............................0.5 g

CuSO . 5H O .......................0.016 g

4 2 Na2MoO4 . 2H2O......................0.025 g

CoCl2 . 6H2O .......................0.046 g

Distilled water....................1.0 L

1% TYE:

Tryptone (BD 211705)..............10.0 g

Yeast extract.....................10.0 g

Distilled water....................1.0 L

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