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ATCC medium: 550 R 8 A H medium

中國微生物菌種查詢網(wǎng) / 2016-11-24 10:22:27

Malic acid.........................2.5 g

Yeast Extract (BD 212750)..........1.0 g

(NH4)2SO4...........................1.25 g

MgSO4 . 7H2O .......................0.2 g

CaCl2 . 2H2O .......................0.07 g

Ferric citrate.....................0.01 g

EDTA...............................0.02 g

KH2PO4 .............................0.6 g

K2HPO4 .............................0.9 g

Trace Elements (see below).........1.0 ml

Vitamin Solution (see below).......7.5 ml

Distilled water to.................1.0 L

Neutralize malic acid with NaOH and adjust the pH of the completed medium to 6.9.

Autoclave at 121C for 15 minutes.

Trace Elements:

Ferric citrate.....................0.3 g

MnSO4 . H2O ........................0.002 g

H3BO3 ..............................0.001 g

CuSO4 . 5H2O .......................0.001 g

(NH4)6Mo7O24 . 4H2O..................0.002 g

ZnSO4 ..............................0.001 g

EDTA...............................0.05 g

CaCl2 . 2H2O .......................0.02 g

Distilled water..................100.0 ml

Vitamin Solution:

Nicotinic acid.....................0.2 g

Nicotinamide.......................0.2 g

Thiamine . HCl.....................0.4 g

Biotin.............................0.008 g

Distilled water....................1.0 L

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