Peptone......................5.0 g
Yeast extract................1.5 g
Glucose......................5.0 g
NaCl.........................1.5 g
Na2HPO4 . 12H2O...............1.5 g
MgCl2 . 6H2O .................0.1 g
Na2CO3 .......................5.03 g
Agar (if needed)............15.0 g
Distilled water..............1.0 L
Sterilize glucose and Na2CO3 separately and adjust final pH to 10.5- 11.0. Autoclave at 121C for 15 minutes.