亚日韩在线中文字幕亚洲,国产在线播放鲁啊鲁视频,张柏芝手扒性器全部图片,亚洲成无码电影在线观看

ATCC Medium: 1439 Methanogenium Medium

中國微生物菌種查詢網(wǎng) / 2016-11-24 12:19:33

NaCl…………………………………………..…….18.0 g

MgCl2 x 7H2O…………………………………..…..2.75 g

KCl…………………………………………..………0.335 g

MgSO4 x 7H2O………………………………..……3.45 g

NH4Cl………………………………………..……...0.25 g

CaCl2 x 2H2O………………………………...……..0.14 g

K2HPO4……………………………………..………0.14 g

Trace Elements (see below)………………..……10.0 ml

Vitamin Solution (see below)…………………….10.0 ml

Fe(NH4)2(SO4)2 x 7H2O……………………….….2.0 mg

NaHCO3………………………………….………...5.0 g

Na Acetate…………………………………..……..1.0 g

Yeast Extract……………………………….……..2.0 g

Trypticase…………………………………….……2.0 g

Resazurin…………………………………………1.0 mg

Cysteine HCl x H2O……………………….………0.5 g

Na2S x 9H2O……………………….………………0.5 g

DI Water to…………………………………….…...1000 ml

This medium has problems with precipitate. Add chemicals in the order as listed. Make mg/ml solution for Fe(NH4)2(SO4)2 x 6H20, Resazurin, and Na2S x 9H20. Gas under 80% N2 and 20% CO2 for 45 minutes. Check pH and lower to 7.0. Dispense, then autoclave.

Vitamin Solution

Biotin………………………………………….2.0 mg

Folic Acid……………………………………..2.0 mg

Pyridoxine x HCl…………………………….10.0 mg

Thiamine x HCl………………………………5.0 mg

Riboflavin……………………………………..5.0 mg

Nicotinic Acid…………………………………5.0 mg

Ca pantothenate……………………………..5.0 mg

Vitamin B12……………………………………0.01 mg

p-Aminobenzoic Acid………………………..5.0 mg

Thioctic Acid………………………………….1.0 mg

DI Water………………………………………1000 ml

Filter Sterilize

Trace Element Solution SL-6

ZnSO4 x 7H2O………………………………..0.1 g

MnCl2 x 4H2O………………………………...0.03 g

H3BO3…………………………………………0.3 g

CoCl2 x 6H2O………………………………..0.2 g

CuCl2 x 2H2O………………………………..0.01 g

NiCl2 x 6H2O………………………………...0.02 g

Na2MoO4 x 2H2O……………………………0.03 g

DI Water……………………………………..1000 ml

Filter sterilize

上一篇：ATCC medium: 1701 Agrobacterium medium

下一篇：ATCC Medium: 8 Nutrient Agar/Broth w/ 3% NaCl