α-Ketoglutarate…………………………2 g
ACES buffer………………………….10 g
KOH…………………………………..3.75 g
Agar (if necessary)………………….15 g
DI Water…………………………….550 ml
Adjust pH to 6.9 and autoclave at 121C.
After cooling, aseptically add:
Fetal Bovine Serum…………………150 ml
TMN-FH Medium (Sigma T1032)…..300 ml