ATCC medium: 1160 Dubos agar with filter paper
NaNO3 ..............................0.5 g
K2HPO4 .............................1.0 g
MgSO4 . 7H2O .......................0.5 g
KCl................................0.5 g
FeSO4 . 7H2O .......................0.01 g
Agar, Bacto (BD 214050)...........15.0 g
Distilled water....................1.0 L
Adjust pH to 7.2 after autoclaving. Sterile strips of Whatman No.1 filter paper are laid on surface of agar. A strip of filter paper is inserted in each tube of broth.