Trypticase Peptone (BD 211921).....5.0 g
Yeast extract......................3.0 g
Sodium lactate (60% solution).....15.0 g
Sodium thioglycollate..............0.75 g
Tween 80...........................1.0 g
Glucose............................1.0 g
Distilled water....................1.0 L
Adjust pH to 7.5 with solid K2CO3.
Autoclave at 121C for 15 minutes.