ATCC medium: 1034 Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
Peptone, Bacto (BD 211677)..............10.0 g
Yeast Extract (BD 212750)...............10.0 g
Yeast nucleic acid*......................1.0 g
Folic acid..............................15.0 mg
Hemin....................................1.0 mg
Distilled water........................880.0 ml
Autoclave for 15 minutes at 121C.
Aseptically add: Buffer Solution (see below).............20.0 ml
Fetal bovine serum (heat-inactivated...100.0 ml
Adjust final pH to 6.5.
* Ribonucleic Acid, Type VI from Torula Yeast, Sigma # R-6625.
Buffer Solution: KH2PO4 ..................................18.1 g
Na HPO .................................25.0 g
2 4 Distilled water..........................1.0 L
Adjust final pH to 6.5.