ATCC medium: 1293 Brain heart infusion-supplemented (BHIS)
Brain heart infusion broth (dehydrated).............37.0 g
Yeast extract........................................5.0 g
Distilled water......................................1.0 L
Resazurin solution (25 mg/100 ml distilled water)....4.0 ml
Boil, cool, add:
L-Cysteine . HCl...................0.5 g
Hemin Solution (see below)........10.0 ml
Vitamin K1 (see below).............0.2 ml
Hemin Solution: Hemin.............................50.0 mg
N NaOH.............................1.0 ml
Distilled water to...............100.0 ml
Vitamin K1 Solution: Vitamin K1.........................0.15 ml
95% Ethanol.......................30.0 ml