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ATCC medium: 535 PYGM medium

中國(guó)微生物菌種查詢網(wǎng) / 2016-12-12 08:24:31

ATCC medium: 535 PYGM medium

Peptone..................................5.0 g

Yeast extract............................2.5 g

Glucose..................................1.25 g

Maltose..................................1.25 g

Cysteine.................................0.125 g

Salts Solution (see below)..............10.0 ml

Resazurin (0.025% aqueous solution)......1.0 ml

Distilled water........................250.0 ml

Autoclave at 121C for 15 minutes. Salts Solution:

K2HPO4 .......................0.1 g

KH2PO4 .......................0.1 g

NaHCO3 .......................1.0 g

NaCl.........................0.2 g

CaCl2 (anhydrous) ............0.02 g

MgSO4 ........................0.02 g

Na2MoO4 . 2H2O............Trace

CoCl2 . 6H2O .............Trace

50% H2SO4 ....................0.3 ml

Distilled water............100.0 ml

上一篇：ATCC medium: 217 Van Niel's yeast agar with 25% NaCl

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