ATCC medium: 66 Acetobacter medium
Autolyzed yeast.............10.0 g
CaCO3 .......................10.0 g
Agar........................15.0 g
Glucose......................3.0 g
Distilled water..............1.0 L
Sterilize. Mix CaCO3 thoroughly and cool rapidly. Slant as the agar hardens so as to keep the CaCO3 in suspension.