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ATCC medium: 2008 TYM medium

中國(guó)微生物菌種查詢(xún)網(wǎng) / 2016-12-16 14:59:47

ATCC medium: 2008 TYM medium

Tryptone (BD 211705)........10.0 g

Yeast extract...............10.0 g

K2HPO4 .......................0.5 g

KH2PO4 .......................0.5 g

NaCl.........................1.0 g

MgSO4 ........................0.1 g

CaCl2 ........................0.1 g

NaHCO3 .......................6.0 g

L-Cysteine . HCl.............0.3 g

Distilled water..............1.0 L

Add components through calcium chloride to distilled water. Heat to boiling and cool in an atmosphere of 100% CO2. Add sodium bicarbonate and cysteine and equilibrate under the same gas phase for 5-10 minutes. Dispense anaerobically under the same gas phase. Autoclave at 121C for 15 minutes. Final pH 6.7 - 6.8.

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