ATCC medium: 2011 Picrophilus medium
(NH4)2SO4.....................0.2 g
MgSO4 ........................0.5 g
CaCl2 . 2H2O .................0.25 g
KH2PO4 .......................3.0 g
Yeast extract................2.0 g
Distilled water..............1.0 L
Adjust for final pH 0.7 with H2SO4. Autoclave at 121C for 15 minutes.