ATCC Medium: 2746 Autoinducer Bioassay (AB) Medium
Base Medium NaCl…………………………………………….....17.5 g
MgSO4………………………………………...…..12.3 g
Casamino Acids………………………………….2.0 g
DI Water…………………………………………..970 ml
Dissolve ingredients and bring the pH of the solution to 7.5 with 3N NaOH. Autoclave base at 121?C and after completely cooling down, add the following ingredients from separate sterile stocks:
Stock Solutions 1M Potassium Phosphate (pH 7.0)………………10 ml
0.1M L-arginine…………………………………….10 ml
Glycerol………………………………………..…….10 ml