ATCC Medium: 2814 YP/10 Medium
Yeast extract…………………………..0.3 g
Peptone……………………………….0.06 g
Tris………………………………….….0.605 g
DI Water……………………………….990 ml
Adjust pH to 7.5
Autoclave at 121C and aseptically add:
200 mM CaCl2 x 2H2O……………….10 ml
Dispense into appropriate vessel.